Biospecimen storage is crucial for scientific research, as it forms the basis of experiments, studies, and medical advances. Proper storage is essential for ensuring the integrity of samples and preventing contamination, degradation, or loss of efficacy. Factors affecting biospecimen stability include temperature, humidity, light exposure, contamination, and mechanical stress. Choosing the right storage solutions, such as cryogenic, ultra-low-temperature freezers, or standard refrigeration, is essential. Regulatory and compliance considerations are also crucial. Best practices for biospecimen storage include accurate labeling, environmental monitoring, inventory management, standard operating procedures, regular maintenance and calibration, security and access control, emergency response planning, and ethical considerations. Technological advancements and sustainability will shape the future of biospecimen storage, with emerging solutions such as dry-state or vapor-phase storage and biodegradable storage components. Case studies demonstrate the importance of rigorous biospecimen storage practices in achieving successful research outcomes.